FOOD SCIENCE ›› 2024, Vol. 45 ›› Issue (7): 332-339.doi: 10.7506/spkx1002-6630-20230813-093
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FAN Lihua, WANG Jiangxue, OU Zongyuan, ZHANG Yanru, Zhang Luyao, LIU Shiyi, ZHOU Zimei, LI Guoliang
Online:
Published:
Abstract: Fecal microbial contamination in the food chain is a significant concern in the field of food safety as it is one of the most important pathways causing foodborne diseases. Microbial source tracking (MST) is an effective approach to address this issue, which can track the sources and contamination of fecal microbes. MST methods can be categorized into two types: library-dependent methods (LDMs) and library-independent methods (LIMs), with LIMs including culture-dependent and culture-independent methods. The non-culture-dependent method using 16S rRNA and host-microbial interaction genes as specific molecular markers has been widely used to investigate microbial contamination in the food chain. This review provides an overview of the basic principle and characteristics of MST, and focuses on recent advances in MST methods based on host-specific molecular markers for tracing microbial contamination in the food chain. Furthermore, future prospects of MST are discussed based on its unique advantages and disadvantages.
Key words: microbial source tracking method; foodborne diseases; fecal contamination; library-dependent; library-independent; host-specific molecular markers
CLC Number:
TS207.4
FAN Lihua, WANG Jiangxue, OU Zongyuan, ZHANG Yanru, Zhang Luyao, LIU Shiyi, ZHOU Zimei, LI Guoliang. Research Progress on Microbial Source Tracking for Traceability of Pollution Sources in Food Chains[J]. FOOD SCIENCE, 2024, 45(7): 332-339.
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URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-20230813-093
https://www.spkx.net.cn/EN/Y2024/V45/I7/332