Abstract:  The Oxygen-resistant fermentation conditions of B-infantis was studied by screening and acclimating in skim milk culture medium. Several optimal promoting substances were obtained from many experiments, which had excellent simulating effect on B-infantis multiplication in oxybiotic process, finally the higher becterial produtive culture medium was gained. So that it was possible to apply this strain made fermented products. Optimal fermentation process was selected by experiments. Bulk starter were prepared by useing the BAT mix culture, or cultivating the B-infantis, S. thermophilus, L. acidophilus individually, then mix them in different proportion. It was normal condition that Bifido yoghurt was produced useing the bulk starter. Not only the taste and aroma of this product are quite similar to original yoghurt, but also it contains 107-8 viable cells of both B-infantis and L. acidophilus bacteria per milliliter. Therefore nutrition value and health care of Bifido-yoghurt are much higher than original yoghurt.

Key words: B-infantis Bifido-yoghurt