Abstract: The method to determin the major Carboxylic acids, Sugars, glucerol and ethanol in wine and grape must is developled using an ion-exchange column and refractive index detector. A solid-phase extraction method with a strong anion exchanger is used to determine these compounds. The method is validated by determining the repeatability, reproducibility, correlation coefficients, recovery efficienty and accuracy and was compared with different methods of analysis.

Key words: High-performance liquid chromatography Analysis of wine Refractive index detector