Abstract: Hot water,hot aqueous alkali, ferrous sulfate aspergillus oryzae were used to degossypol from cottonseed cake.The optimum result came from the use of hot aqueous alkali.After orthogonal experiment the optimum condition was pH8 ~ 9.temperature 60℃.tirne 3hr. and the tinal gossypol content of aqueous cotton seed protein was 12ppm.This content was much lower than the standard of free gossypol content of edible cottonseed protein stipulated by UNCC.

Key words: Cottonseed Gossypol Degossypol