Abstract: Extraction of Mucopolysaccharides from shark fin cartilage by digested with trypsinase was studied.The increase of heating pretreatment could improve the hydrolysis of shark fin cartilage and increase the content of mucopolysaccharides and sulfate in the extraction in the range 70℃to 90℃.The effects of temperature, pH, enzymatic addition and time of hydrolysis of shark fin cartilage with trypsinase were investigated and the optimum hydrolysis condition was as follows:with 3% of enzymatic addition at 50℃and pH 8.0 for 26h. Among chloroform-butyl alcohol (9:1),chloroform-Pentanoic alconol (9: 1) and trichloroacetic acid,trichloroacetic acid was the best protein precipitant for isolation of shark fin cartilage mucopolysaccharides.

Key words: Trypsinase Mucopolysaccharides Shark fin cartilage Hydrolysis Extraction