Abstract: By using a special edible fungi,Dictyophora indusia Fisscher, a kind ofantimicrobial that could be added into food was extracted. By means of filter paper disks tests. the antimicrobial actions of Dictyophora indusia Fisscher’sextract were verified.And then the extract’s antimicrobial efficency in Bacillus subtilis and Bacillus cereus was taken as the measurements in this experiment.Through orthogonal design, the optimal extract process was obtained. And the extract’s antimicrobial effects on the major pathogenetic bacteria and spoilage bacteria in food were also obtained.The results showed that the extract from Dictyophora indusia Fisscher had obvious antimicrobial actions on 8 kinds of pathogenetic bacteria and spoilage bacteria. It showed strong antimicrobial effects under neutral and weakly basic conditions.

Key words: Dictyophora indusia Fisscher antimicrobial action Orthogonal design Variance analysis