Abstract: The ice-nucleation active (INA) bacteria (Pseudomonas syringae QF-95-F19) of the high-level expression ice-nuclei feature under the different conditions was studied in this paper. Culture methods were used in 22℃ and 32℃shifted to 18℃ as two segment temperature cultures , TSTC with the optimal growth medium(M1) as the basic medium to do the experiment. The effects of different temperatures on high-level expression of ice-nuclei were detected assayed in the M1. Experiment results confirmed that low temperature was a required factor for expressing ice-nuclei in TSTC and ice nucleation protein became necessary . From the effects of the different pH on the high-level expression ice nuclei to QF-95-F19, the relationship between the pH and ice nuclei tromation and the growth situation of the bacterium was determined further. It confirmed that the different inhibitors had inhibition remarkably for the expression of QF-95-F19 ice nuclei and its growth by this experiment.

Key words: Ice-nucleation active (INA)bacteria Ice-nuclei Two segment temperature culture (TSTC) method Inhibitor Induction