Abstract: CPPs (Casein phosphopeptides)are mulitibioactive peptides. There are many research works done in many countries for CPPs preparation, but up to now, what was used to produce CPPs in large scale was trypsin.However, it is known that trypsine was very expensive. So in this paper, a much more cheaper proteasae, alkaline protease 2709 was found to be suitable for CPPs preparation in large scale.

Key words: alkaline protease CPPs Casein preparationh