Abstract: Comparison study was made on the four factors affecting the amount of autolysate of Se-rich yeast, namely: kinds of the culture medium, Se enriching time, different Se concentrations and Se added according to the degree of concentration. On the basis of the total Se-concentration limited to -15μg/ml, the optimal Se-autolysates were obtained with adding time as 10, 12, 14, 15, and 16h, corresponding to adding concentration of Na2SeO3.as 1, 2, 3, 4 and 5μg/ml respectively. The optimal Conditions for the autolysis of Se-rich Brewer’s yeast were: 3%NaCl, pH5.5, 55 ℃ and 20h.

Key words: Rich-selenium Brewer &rsquo, s yeast leakage components Add selenium conditions The amount of Se Autolysis conditions The amo of funtree &alpha, -amino nitrogen；