FOOD SCIENCE ›› 2000, Vol. 21 ›› Issue (2): 57-59.
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HE Xin-Xia, ZHENG Xiao-Hua, JIA Hai-Yan, YE Feng
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Abstract: A simple rapid and accurate quantitative method was established for the determination of lecithin in soybean phospholipids by HPLC.Separation was obtained by using Lichrosorb si60 column (5 m250 x 4mm ) and mobile phase of acetonitrile:methanol:water (65:21:14v/v) with flow rate of 1.8ml/min .The eluates were monitored with UV detector at 205nm.The retention time for lecithin was 4.875min .The lecithin curve was linear in the range of 0.04 0.08mg/ml with =0.9996.The average recovery rate for lecithin was 98.35%.RSD=6.94%(n=6).
Key words: Soybean Phospholipid lecithin HPLC
HE Xin-Xia, ZHENG Xiao-Hua, JIA Hai-Yan, YE Feng. Quantitative Analysis of Lecithin in Phospholipids by HPLC[J]. FOOD SCIENCE, 2000, 21(2): 57-59.
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