FOOD SCIENCE ›› 2000, Vol. 21 ›› Issue (2): 60-62.

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Simultaneous Determination of VC and L-cys in Fruit Juices by Stopped-Flow Kinetic Spectrophotometry

 ZENG  Sa, TAN  Hui-Ling   

  • Online:2000-02-15 Published:2011-11-25

Abstract: Astopped-flow kinetic spectrophotometric method is proposed for the simultaneous determination of ascorbic acid (Vc ) and L-cysteine(L-cys)in fruit juices.The calibration graphs are linear for 0-24.0-280mg/L and the detection limits are lmg/ L and 16mg/L for Vc and L-cys.respectively.The injectd sample volume is 157uL and the sampling rate is 30 samples/h.This method is simple rapid. sensitive and accurate with high selectivity and has been applied for determination of Vc and L-cys in strawberry juice.The marked recovery rates are up to 95.2%and 92.8%for Vc and L-cys respectivily.

Key words: Stopped Folw Analysis Simultaneous Determination Spectrophotometric method Ascorbic acid L-cysteine