Abstract: Calpain and calpastatin system would play all important role in muscle growth.Myoblast differentiation and fusion in multinucleated muscle cells was studied in myoblasts growth in culture The content of calpastatin in muscle would be related to cell membrane fusion.Myoblast fusion would required calpain activation while its fusibility would depend on the ratio of cell calpain to calpastatin.In the present study. we found that EDTA. EGTA and GET-calpastatin (IV ) inhibited myoblast fosion.GST-calpastatin (IV).a 45KD fused protein could be induced to express by 0.4mmol/L IPTG.When the concentrations of GST-calpastatin (IV) were 2.5 ul/ml and 0.5 ul/ml.cell fusibility were 5% ~ 10% and 10% ~ 15%, respectively.

Key words: Calpain Calpastatin, Cell Fusion Inhibitors