Abstract: Pregnant cows were immunized during their late gestation with Human Rotavirus (R.V) and Escherichiacoli (E.coli) which would cause diarrhea in human being and induce hyperimmune antibod ies in cows The antibody levels in milk were detected by In vitro Agglutination and Reverse Indirect Hemagglutination Inhibition Test and their changes were described.It showea that the levels of hyper immune antibodies in milk resistant to E.coli and R.V.were maintained for two months, and could be maintained for another month when the cows were immunized again.

Key words: Immune milk Escherichiacoli (E.Coli) Rotavirus (R.V) Antibodies maintaining