Abstract: In this paper,a simple, rapid and accurate HPLC method for the simultaneous determina tion of α-γ-δ-tocopherol, the three vitamin E isomers, has been established.The separation was achieved by using ALLTIMA C18 （4. 6 × 250mm） as chromatogram column and methonol/water=98. 5/1. 5 as mobile phase. It was edtected at 296nm by photodiode array detector.The retention time of the three isomers were 13. 6237min, 11. 2615min and 8. 9608min, and their detecting limitation were 5ng、3. 5ng and 1. 4ng resectively. The contents of α-γ-δ-tocopherols in wheat germ were 41. 45 ± 1. 21mg/100g, 10. 13 ±0. 42mg/100g and 3. 79±0. 28mg/100g respectively.

Key words: Tocopherol HPLC Determination