Abstract: Among the mediums MRS, M17, ATP, SL, Elliker, SDM used for lactic acid bacterium growth, a new culture medium ATP was chosen for the synthesis of exopolysaccharide (EPS) by Streptococcus salivarius subsp. thermophilus LCX2001. When grown in a chemically defined medium with glucose and/or lactose as the substrate carbohydrate added in the amount of 20g/L. The production of EPS increased at higher temperatures, but the bacterium rapidly lost its EPS producing cap ability at 47℃, while the optimization temperature was 35℃,initial pH of medium was 6.5, and the fermentafion time was 20h.

Key words: Exopolysaccharide Medium Synthesis Lactic Acid Bacteria