Abstract: The scavenging effect of LBP2 (one component of polysaccharides from Fructus Lycii,the fruit of Lycium barbarum L.) on hydroxyl radicals (·OH) was studied by salicylic acid method.To study the anti-oxidative effect of LBP2 on ·OH,the content of MDA in liver was measured by TBA assay ,the mitochondria membrance fluidity in liver was measured by the fluorescence polarization assay with DPH fluorescence probe,and the hemolysis extent of red blood cells and the swelling extent of mitochondria of liver were detected by spectrophotometric methods.The result showed that LPB2 had the ·OH scavenging activity.It could inhibit the generation of MDA,the decrease of mitochondria fluidity,the swelling of mitochondria,and the hemolysis of red blood cells induced by ·OH .The conclusion was that LBP2 had the effect of anti-·OH induced oxidation.

Key words: Lycium barbarum L. Polysaccharide Hydroxyl radical Anti-oxidation