Abstract: Enzymolysis test about the conchiolin extracted from the shell nacre of pearls by using pineapple protease and papain was made to examine the UV absorbance of peptide and tyrosine at the wavelength of 275 nm with ultraviolet spectrometry.After observing and analyzing conchiolin enzymolysis effect of the two kinds of plant protease,we probed into the conditions of its enzymolysis.The conclusion showed.The two kinds of plant protease have had particular enzymolysis effects and the enzymolysis effect of pineapple protease was higher than the papain’s.It appeared a line relation ship with enzymolysis products when the substrate concentration was less than 0.2%.It was optimal when the enzyme concentration was kept under 100μg/ml and enzymolysis time about 10 hours.

Key words: Conchiolin Pearl shell Pineapple protease Papain