FOOD SCIENCE ›› 2001, Vol. 22 ›› Issue (2): 64-67.
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ZHAO Ren-Bang, GE Wei, CUI Tong, LIU Shu-Chen, ZHANG Dong
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Abstract: Using 70% methanol, the Polygonum multiforum extract was concentrated by ultrasonic method .Then concentrated extract was leached with chloroform and separated into free and bound anthraquinones.The mobile phase was V(CH3OH):V(C3H7OH):V(H2O)=80:10:10 (control pH to 3.0 with phosphorus acid ).The HPLC system was consisted of Hypersil BDS C 18(5μm,250nm×46nm) and UV detector set at 439nm.The experiment results show that the average recovery of Emodin was 100.7% (RSD=1.77),and that of Emodin methyl...
Key words: HPLC Polygonum multiforum Anthraquinones Emodin acid Emodin Emodin methylether
ZHAO Ren-Bang, GE Wei, CUI Tong, LIU Shu-Chen, ZHANG Dong. Determination of Emodin Acid, Emodin and Emodin Methyl-ether in Polygonum multiforum by HPLC[J]. FOOD SCIENCE, 2001, 22(2): 64-67.
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