Abstract: A method for determination of fumonisins B1 and B2 in beer by High Performance Liquid Chromatography was reported.The sample was cleaned up by fumonitest affinity cartridges. Fluorescent fumonisins derivatives were formed by adding OPA, separated on Spherisorb C18 column , detected by fluorescent detector and quantified by external standard. Beer samples were fortified with a misture of fumonisins B1and B2 at the four levels.Five replicate analysis were performed at each level .The recoveries of the fumonisins were 86.1%-93.4% for B1 and 71.6%-82.0% for B2.The variation coefficients were 4.8%～7.2% for B1 and 6.3%～8.9% for B2.The detecttion limits were 0.005mg/kg for B1 and 0.01mg/kg for B2.

Key words: Fumonisins Beer Affinity Cartridges HPLC