FOOD SCIENCE ›› 2001, Vol. 22 ›› Issue (3): 26-29.

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Study on Extraction of an Edible Fungus Ploysaccharide and its Effect of Cleaning Oxygen radicals

 LI  Xue-Hua, LONG  Sheng-Jing   

  • Online:2001-03-15 Published:2012-02-15

Abstract: To isolate and extracte an edible fungus (Auricularia auricula-judae)polysaccharide (AAPS)and analyse its effect on cleaning all kinds of oxygen radicals,a technique of hot water extract,ethanol sedimentation,chloroform and butanol deproteinization was preformed.Sugar content was also determined.With the help of chemiluminescent analysis,AAPS was separately determined for cleaning up oxygen free radicals of H2O2、O·-2、·OH.The extraction rate of AAPS was 0.748%.Total sugar content was 74.67%.AOV was determined respectively as 105.324 for ·OH 34930.795 for O·-2,606.5 for H2O2,and 0.859 for oxygen free radicals from phagocytic of leucocyte in whole blood Chemiluminescence.The test showed that AAPS could weed out oxygen radical, and its effect increased with increasing amount of sugar. AAPS had the strongest ability in clearing up oxygen free radicals from chemiluminescence in whole blood under the circumstance of whole blood physiology.

Key words: An edible fungus Polysaccharide Oxygen free radical Chemiluminescent Isolation Extract