Abstract: In this study,alcalase was used for producing CPP by hydrolyzing casein.The optimum conditions:casein concentration 10％ ,enzyme usage 1500u/g substrate,temperature 45℃ ,pH10.5 and time 150mins were determined.A selective precipitation procedure was also used to isolate CPP from the hydrolyzate of casein.The N/P ratio and the yield of CPP obtained by adding 1.1％ CaCl 2(w/v) and 50％ (v/v) alcohol to the hyrolyzate at pH4.5 were 6.39％ and 13.94％ respectively.

Key words: Casein phosphopeptide 2709 alcalase Casein