Abstract: Continous fermentation of multimicrobe had been performed by using immovable cell culture.We simulated cellar fermentation of liquor by using 30 fermentation collumns,which represented different fermentation stages.By controlling tempratures artificially and changing culture components,we detected the total easter,total acid,alcohol,pH value and changes of different organic acids under different conditions and stages by using gas chromatograph.The results showed that the produce of butyric acid culminate in the column 19,while that of caproic acid in the column 22.The number of the microbe was maximun in the column 7,while on the column 19,it was minimum,following an ascend trend.In the firmenting columns,Ananerobic Bacillaceae was the dominant strains.In the experiment,we analysized the organic acid-producing of the immobilized multimicrobe by using the extract liquid of the fresh distiller’s grains as the basic fraction,the results was good.

Key words: Immobility , Continuous fermentation , Arganic acid , Microbe