Abstract: Through the orthogonality experiment, the optimization culture condition for accumulating CBE by Mucor circinelloides 3.2208 was achieved as following:inoculum quantity 0.5ml;temperature 33℃;pH6.0;C/N ratio100;culture time 6 days;nitrogen source is peptone;carbon source is maltose;FeSO4•7H2O 0.01g/L;ZnSO4•7H2O 0.01g/L;MnSO4•4H2O 0.0005g/L;MgSO4•7H2O 0.8/L;Oleic 0g/L.Under such culture condition,we gain the Mucor circinelloides 3.2208 CBE 3.61%from dry cell.The lipid coefficient is 0.29,the Sn-2unsaturated fatty acid is up to 78.08%.

Key words: Mucor circinelloides 3.2208 , Cocoa Butter , Equivelent Optimal culture condition