Abstract: We seperated and purified fine microphyte strains from fermentation pit soil and studied the organic acid-producing results by using immobilized cell culture.We analysized the changes of organic acid by gas chromatograph when we changed the culture components and other conditions.The results were as follows:Fornic acid and valeric acid restrained the acid-producing while sodium acetate showed a direct plus correlation to that of the multimicrobe.The carbon as nutritent source was composed of a mixture of acetic acid and lactic acid,acetic acid and propionoic acid,acetic acid and butyric acid and propionoic acid and butyric acid so as to improve organic acids-producing.In addition,P and N had also a important effect organic acid-producing.However,the total trend of organic acid-producing was as follows:Acetic acid→butyric acid→acproic acid.In the experiment,we analysized the organic acid-producing of the immobilized multimicrobe by using the extract liquid of the fresh distiller’s grains as the basic fermentation fraction.The results were good,and might be utilized in the production of Luzhou-flavour liquors.

Key words: Immobilized multimicrobe Fermentation Arganic acid-producing