Study on Isolation,Purification,Analysis of the Components and Anti-Oxidation Activity of thePolysaccharides of the Fruits of Hippophae rhamnoides

Abstract

Abstract: The polysaccharides of Hippophae rhamnoides were isolated in hot water,and precipitated with perpendicular experiments.The protein in polysaccharides was removed by Sevage way.The polysaccharides were graded to A.B.C and D by acidic ethanol.C was further purified to C1 and C2 with DEAE column chromatography.Sephadex G-75 column chromatography and polyacrylamide gel electrophoresis provde that C1 was one polysaccharide and C2 was two polysaccharides.Their components were analyzed with thin-layer chromatography and the results were that there was glucose in C1 and there were wylose and other unknown monosaccharide in the main component of C2.The anti-oxidation experiments indicated that the polysaccharides of Hippophae rhamnoides had anti-oxidation activty and this activity was increased with the inereased concentration of the polysaccharides.

Key words: Hippophae rhamnoides , L. Polysaccharides , Isolate , Purify

FU Ming-Hui, LIN Zong-Hua. Study on Isolation,Purification,Analysis of the Components and Anti-Oxidation Activity of thePolysaccharides of the Fruits of Hippophae rhamnoides[J]. FOOD SCIENCE, 2002, 23(3): 73-75.

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Study on Isolation,Purification,Analysis of the Components and Anti-Oxidation Activity of thePolysaccharides of the Fruits of Hippophae rhamnoides

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