Abstract: The quantity of Ginkgo Flavonol Glycosides in GBE （Ginkgo Biloba Extract） was determined by FPLC techniques using external standard method. The column was packed with C18 (4.6μm×250mm). The mobile phase consisted of method-phosphate-water(60:5:35).The flow rate was 1.5ml/min and detection was affected at 360nm. It showed that the quantity of sample and the area of peak were in linear relationship from 2μg/ml to180μg/ml. The average recovery was 97.5%. The relative standard deviation was 1.60%. After the hydrolysis of the flavonoids , the content of quercentin decreased, but the content of kaempferol increased and isorhamnetin remained the same level.

Key words: FPLC , Flavoinds quercetin , Extract of ginkgo biloba L