Abstract: The optimal condition of Chlorella ellipsoidea L1 strain under heterotrophic culture to get high biomass were: BG-11 medium with 10g/L of glucose as the carbon source and 0.2-1g/L of Urea as the nitrogen source,25～30℃ cultivation temperature, initial pH 5.5～6.5, 100ml medium in 250ml flask and seeding 10%(V/V). By inoculating the pre-culture to a conventional 2L aerated and stirred the minijar ferment containing 1.5L of the medium, we got dry cell weight 5.42g/L. The productivity was about 20 times as much as that for photoautotrophically grown cultures. Compared with phototrophic, the lipids content increased, whereas the protein contents and ashes decreased.

Key words: Heterotrophic cultivation , Autotrophic cultivation , Chlorella ellipsoidea