Study on PCR-ELISA for the Detection of Genetically Modified Organisms Products

FOOD SCIENCE ›› 2003, Vol. 24 ›› Issue (1): 101-105.

Study on PCR-ELISA for the Detection of Genetically Modified Organisms Products

LIU Guang-Ming, XU Qing-Yan, LONG Min-Nan, SONG Si-Yang, ZHOU Jun, SU Wen-Jin

Online:2003-01-15 Published:2011-12-13

Abstract

Abstract: The methods of extracting DNAfromgenetically modified organisms (GMOs )were studied and ob-timized.According to the characteristics of CaMV 35S promoter&T -NOS te rminator introduced into GMOs ,PCR -enzyme linked immunosorbent assay(PCR -ELISA)methods were suitable for screening of transgenic elements andtherefore established.Withthis methodwe analyzedsoybeans andcorns.The results showedthatthemethods of PCR -ELISAwere simple,rapid and reliable for qualitative a nd quantificational analysis of GMOs.

Key words: GMOs, detection technique, PCR -ELISA

LIU Guang-Ming, XU Qing-Yan, LONG Min-Nan, SONG Si-Yang, ZHOU Jun, SU Wen-Jin. Study on PCR-ELISA for the Detection of Genetically Modified Organisms Products[J]. FOOD SCIENCE, 2003, 24(1): 101-105.

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Study on PCR-ELISA for the Detection of Genetically Modified Organisms Products

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