Abstract: The headspace aroma compounds of the powder of Black Piper Nigrum L.were investigated by gas chromatography-mass spectrometry.The headspace volatiles were sampled by solid-phase microextraction(SPME).SPME sampling was con-ducted with 30μm PDMS fiber at 25±5℃ for 40min.The fibers were desorbed in a GC injection liner at 250℃for 5min.ACapillary column(WCOT FUSED SILICA 30m×0.25mm ID COATING CP-SIL 8 CB-LOW BLEED/MS DF=0.25μm)wasemployed in GC/MS analysis.Initial oven temperature was kept at 40℃ for 5min,then raised to 250℃ at 3℃•min-1,and keptat 250℃ for 5min.The chemical constituents of three samples of Black Piper Nigrum L.produced by different companies wereseparated and identified by GC-MS.A total of 53 volatile compounds were identified and the β-Caryophyllene,3-Carene, β-Phellandren,α-Copaene,δ-Elemene,β-Pinene and α-Humulene were the most abundant aroma compounds from the powdersof Black Piper Nigrum L. In this study,headspace SPME-GC-MS could afford a simple sampling method for the headspacearoma compounds of the powder of Black Piper Nigrum L.and could be used to distinguish the kinds of powder of Black PiperNigrum L.produced by different companies.

Key words: Black Piper Nigrum L, aroma compound, solid-phase microextraction (SPME), gas chromatography/massspectrometry