FOOD SCIENCE ›› 2003, Vol. 24 ›› Issue (10): 59-62.

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Study on Properties of Polyphenol Oxidase (PPO)and Control of Browning in Dioscores

 CHEN  Yan-Le, SHEN  Xiu-Ying, DING  Bing-Yang, JIA  Shou-Ju   

  1. 1.  2. Department of Biological and Environmental Sciences, Wenzhou Normal College
  • Online:2003-10-15 Published:2011-12-13

Abstract: The polyphenol oxidase has been extracted from Dioscorea. The kinetic characteristics of polyphenol oxidase inDioscorea were studied with spectrophotometer. The results showed that the optimum pH was 5.8 and temperature was 20℃.The value of Km and Vmax was 1.82mol/L and 1.11(ΔOD/min) respectively.The effect of inhibitors on activity of PPO wasstudied in this paper as well.

Key words: dioscorea, polyphenol oxidase, kinetic characteristics, browning