Abstract: In this paper, the technology of Enzyme-Linked Immunosorbent Assay (ELISA) was briefly introduced. Meanwhile,the application of ELISA in analysis of food safety was commented in details, including the determination of food toxin, residualpesticides, food microorganisms, food quality and pollution of heavy metal. Finally, the tendency of development in ELISA andits prospect used in the food safety determination were also forward promoted.

Key words: ELISA, food safety, rapid determination