Study on Isolation and Purification of Nisin Adsorbed to Lactococcus lactis subsp Lactis SQ80

Abstract

Abstract: The adsorption rate of nisin to Lactococcus lactis subsp. Lactis SQ80 at different pH conditions was studied. AtpH 6.5, it was 91.5%, the biggest one, but at pH<3.0, nisin wasn’t adsorbed at all. By changing pH , SQ80 cells could adsorbor release nisin selectively. The efficiency of isolation and purification was high. HPLC showed the single peak.The spelifil activityof nisin was increased 75 fold with a yield of 58.2%.

Key words: Lactococcus lactis subsp. Lactis, nisin, isolation and purification, adsorption rate

WU Qiong, SHENG Ying-Tao, TANG Ming-Shan, GU Ti-Hua, GE Qing-Yan. Study on Isolation and Purification of Nisin Adsorbed to Lactococcus lactis subsp Lactis SQ80[J]. FOOD SCIENCE, 2003, 24(9): 103-106.

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Study on Isolation and Purification of Nisin Adsorbed to Lactococcus lactis subsp Lactis SQ80

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