Study on Effects of Five Carbon to Decolor β-mannanase of Bacillus Subtilis

Abstract

Abstract: The effect of active carbon on theβ- mannanase of Bacillus subtilis was investigated. The results showed that carbonwas found to be very effective for decolorizing crude beta-mannanase. The conditions of using 3% carbon, treated at 50℃ andpH 6.5 for a duration of 2h were optimal for decolorizing and purifying the enzyme. Under these conditions, the enzyme couldbe purified by 3.77±0.15 fold with a yielding recovery of 86.19%±2.99%.

Key words: active carbon, β- mannanase, purification, decolorizing

YU Hong-Ying, SUN Yuan-Ming, YANG You-Hui, YANG Yue-Sheng. Study on Effects of Five Carbon to Decolor β-mannanase of Bacillus Subtilis[J]. FOOD SCIENCE, 2003, 24(9): 106-108.

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Study on Effects of Five Carbon to Decolor β-mannanase of Bacillus Subtilis

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