New Method on Streptomycin Residues Assay in Honey by HPLC

FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (1): 155-158.

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New Method on Streptomycin Residues Assay in Honey by HPLC

CHEN Xiao-Hong, LIU Xiao-Li, DONG Ming-Sheng, CHEN Hui-Lan

1.College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Ministry Agriculture;2.Jiangsu Province Exit/Entry Inspection & Quarantine Bureau

Online:2004-01-15 Published:2011-10-24

Abstract

Abstract: HPLC with post-column derivatisation was used to detect Streptomycin residues in honey, with 0.01mol/l sodium1-Heptanesulfonate and acetonitrile(65+35) as mobile phase and fluorescence detection (Ex=263nm,Em=447nm). Detectionconditions and parameters including the proportion and speed of mobile phase, arrangement of deriving agents, dosage of solutionand elution and usage of sedimentary agent were optimized. A new method with simplicity, repeatability and accuracy wasestablished in this paper.

Key words: streptomycin, HPLC, fluorescence, post-column derivatization

CHEN Xiao-Hong, LIU Xiao-Li, DONG Ming-Sheng, CHEN Hui-Lan. New Method on Streptomycin Residues Assay in Honey by HPLC[J]. FOOD SCIENCE, 2004, 25(1): 155-158.

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New Method on Streptomycin Residues Assay in Honey by HPLC

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