Development of PCR Detection Method for Geneticcally Modified Component in Familiar Edulis Fungi

FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (10): 206-210.

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Development of PCR Detection Method for Geneticcally Modified Component in Familiar Edulis Fungi

CHEN Wen-Bing, JIANG Shu-Xun, SHAO Bi-Ying, LI Shou-Song, ZHU Xiao-南, WANG Ze-Sheng, LIAO Jian-Hua

1.Fujian Entry-exit Inspection and Quarantine Bureau;2.Fujian Research Institute of Light Industry

Online:2004-10-15 Published:2011-10-24

Abstract

Abstract: DNA extracted from plasmid vector (p301-bG1) used in transgenic research of familiar edulis fungi was added into19 familiar edulis fungy samples as simulated positive samples. The qualitification PCR was used to detect genetically modifiedcomponent in edulis fungi in present study. DNA extracted from samples by CTAB method was amplified by single PCR andmultiplex PCR. We developed the PCR detection method for the target DNA fragments with the size of 165bp, 398bp and599bp of three transgenes, i.e. NOS, BAR and GUS, respectively. Effect of different DNA concentration of positive sample (p301-bG1 plasmid) to PCR analysis was also analyzed.

Key words: edulis fungi, genetically modified component, PCR detection

CHEN Wen-Bing, JIANG Shu-Xun, SHAO Bi-Ying, LI Shou-Song, ZHU Xiao-南, WANG Ze-Sheng, LIAO Jian-Hua. Development of PCR Detection Method for Geneticcally Modified Component in Familiar Edulis Fungi[J]. FOOD SCIENCE, 2004, 25(10): 206-210.

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Development of PCR Detection Method for Geneticcally Modified Component in Familiar Edulis Fungi

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