FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (10): 247-250.

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Determination of Remained Enrofloxacin in Eel by ELISA

 ZHENG  Jing, HUANG  Xiao-Rong, LI  Yao-Ping, LI  Xiao-Jing, LIN  Jie   

  1. Fujian Province Entry-Exct Inspection and Quarantine Bureau
  • Online:2004-10-15 Published:2011-10-24

Abstract: Remained Enrofloxacin in eel were determined by ELISA. The calibration curve should be virtually linear in the rangeof 0.3~10μg/kg. The limit of detection (LOD) is 3μg/kg. Adding Enrofloxacin to eel samples, the concentration is 25, 50 and100μg/kg separately. Average recovery rate is 74.5%, 76.6%, and 66.5% separately. Coefficient of variation in same batch is7.62%~14.31%. Coefficient of variation in different batch is 6.03%~7.00%. Sensitivity is 0.3μg/kg. The rusult of special testis as following: No cross-reactivities happened to other drugs.

Key words: eel, remained Enrofloxacin, ELISA