Abstract: Eight Pharmacologically Active Phenolic Acids including protocatechuic, gallic acid, caffeic acid, chlorogenic acid,syingic acid, p-coumaric acid, ferulic acid and ellagic acid were identified in buckwheat honey by high-performance liquidchromatography with electrochemical detector(HPLC-ECD). The results showed that the main phenolic acids were gallic acid,p-coumaric acid, protocatechuic, caffeic acid and ferulic acid. Their concentration in buckwheat honey were 461μg/100g honey,150μg/100g honey, 30μg/100g honey, 79μg/100g honey and 39μg/100g honey respectively. Superoxide radical inhibiting,hydroxyl radical scavenging, 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical scavenging and lipid-microsomal peroxidationassay system were used to evaluated the antioxidant activity of buckwheat honey phenolic acids(BWPC). Our results indicatethat BWPC has the capacity to scavenge free radicals and inhibit lipid peroxidation.

Key words: buckwheat honey, phenolic acids, HPLC-ECD, antioxidant activity