Abstract:  A new Biuret reagent and test procedure was introdued in this paper. How proper using of the method to assay proteincontained in Buckwheat was investigated also. The results had showed two facts ① The reagent and the procedure werepracticable for analysis of different samples, with high precision (Cv<0.5%) and fair reclamation (>%). As BSA being thestandard, the analysis result is alkaline soluble protein content of the sample. As the correct representative sample being thestandard, the analysis result is total protein content of the sample. ② The protein content of White Flower Buckwheat kernelwas 11.6%. The protein content of the inner part of the kernel was 5.6%. The alkaline soluble protein content of White FlowerBuckwheat kernel was 7.6%. The alkaline soluble protein content of the inner part of the kernel was 2.5%.

Key words: biuret, buckwheat protein, flower buckwheat