Study on Screening Strain and Cultural Conditions of Strain Obtained by Soybean Isoflavones Glycosides Transform Enzyme

FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (10): 56-59.

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Study on Screening Strain and Cultural Conditions of Strain Obtained by Soybean Isoflavones Glycosides Transform Enzyme

吕Xiao-Lian , FAN Li-Qing, JIA Jian-Hui, TANG Shu-Ju, GUO Hong

Beijing Food Research Institute

Online:2004-10-15 Published:2011-10-24

Abstract

Abstract: Aspergillus niger 3866 is confirmed the highy enzyme activity producing strain by screening strain .The prescriptionof best medium of producing enzyme is: Bran is 4%, (NH4)2SO4 0.1%, KH2PO4 0.1%, ascorbic acid 0. 1%, MgSO4 0.1%,salicinis 0. 05%. The best cultivation condition of producing enzyme is pH 6. 0, 30℃, 160 r/min, 84 h. Enzyme vigor can reach as highas 152.45U.

Key words: Soybean isoflavones, glycosides transform enzyme, screening strain, condition of enzyme production. .

吕Xiao-Lian , FAN Li-Qing, JIA Jian-Hui, TANG Shu-Ju, GUO Hong. Study on Screening Strain and Cultural Conditions of Strain Obtained by Soybean Isoflavones Glycosides Transform Enzyme[J]. FOOD SCIENCE, 2004, 25(10): 56-59.

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Study on Screening Strain and Cultural Conditions of Strain Obtained by Soybean Isoflavones Glycosides Transform Enzyme

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