Abstract: The technique of refining glue polysaccharide from Skate’s cartilage is studied and the content of gluepolysaccharide is measured.The experiment study shows: When the content of NaOH is 6%, temperature is 40℃, extractingtime is 6h,the time of neutral protein enzyme dissolution is 7h under 40℃, Trichloroacetic acid precipitate & separate protein,4 times volume 95% C2H5OH precipitate glue polysaccharide,the extracting rate is over 21.84%, the product is white powderand purity is about 83.52%.

Key words: skate’s cartilage, glue polysaccharide, alkali extracting, enzyme dissolution