FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (7): 128-131.

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Preparation of Mixed Plant Sterol Standard Sample and Its Application in Analysis

 CHEN  Mao-Bin, WU  Mou-Cheng   

  1. 1.College of Food Science and Technology,Huazhong Agricultural University;2.Department of Bioengineering,Hubei Polytechnic University
  • Online:2004-07-15 Published:2011-10-24

Abstract: The preparation and purification of mixed plant sterol standard sample by a recrystallization method were studied.Best solvents and operate conditions were found .The experimental results showed that the total content of plant sterol washigher than 99% and the total yield was higher than 55% after the third recrystallization operation. The applications of mixedplant sterol standard sample in quantitative analysis by gas chromatography were studied. These methods were found convenient,short-cut, cheap and practical.

Key words: plant sterol, standard, recrystallization, gas chromatography