Abstract: A method for economizing the 2,4-Dinitrophenylhydrazine colorimetry of protein carbonyl group was discussed.By the improved method it was possible to analyze the functional health food economically. In this method Aprotininwas substituted by liquid Nitrogen. Both ways were taken to measure the protein carbonyl content in the brain tissueof mice separately in the same age and different ages. Two kinds of antioxidative functional health foods were used totest the feasibility of this new method. It showed that there was no obvious difference(p>0.05)between the resultsobtained by the improved and the classic methods. It revealed that the influence of the anti-oxidation functional healthfood to the carbonyl content in protein could be detected by this improved method.

Key words: evaluate anti-oxidative health food, detection the carbonyl content of protein, improved 2,4-Dinitrophenylhydrazine colorimetry