Abstract: The paper probed into the extraction methods and affective factors on the stability of carotenoids pigments fromthe natural red yolk of duck eggs. The results showed: for extracting the carotenoids pigments of freeze-dried red yolk powderthe optimum conditions were perfomed by petroleum ether: acetone=2:1(V/V), red yolk powder: solvent = 1:4 (W/V), extracting3 times with 60min per time and 40℃; The carotenoids pigments were stable under the condition of low temperature. Thecarotenoids pigments were not stable when K+、Na+、Ca2+、Mg2+、Cu2+、Zn2+、Fe3+ or Al3+ existed; The carotenoidspigments were very unstable under the prosence of Fe2+ or ray, while VC, VE, BHT, or ethyl-P-hydroxybenzoate had a good protecteffect on the carotenoids pigments.