FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (1): 121-124.

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Study on Aqueous Two-phase Systems to Produce Amino Acids Through Gelatin Hydrolysis

 ZHANG  Gang, HUANG  Deng-Yu, ZHANG  Cong-Yue   

  1. Tianjin Research Institute of Industrial Microbiology
  • Online:2005-01-15 Published:2011-09-19

Abstract: s:To produce amino-acids through gelatin hydrolysis, catalyzed by protease by aqueous two -phase systems was studied. The constituted 20% PEG1540 with 10% potassium phosphate by the aqueous two phase system with 3% alkali protease and 35% gelatin added was hydrolyzed at the conditions of pH7.5 and 45℃ for 10h. With the hydrolysis of 83.2%, 76. 7% amino acids was obtained, totally constituted 13 kinds of amino acids.

Key words: two-- phase system, gelatin, hydrolysis, amino acids