FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (10): 196-199.

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Comparison between Polyacrylamide Gel Electrophoresis and Agarose Gel Electrophoresis in Detecting Genetically Modified Soybean

 CHEN  Ji-Cheng, ZHOU  Rui-Bao   

  1. Institute of Soybean Advanced Process, Henan University of Technology
  • Online:2005-10-15 Published:2011-09-25

Abstract: The CaMV35S promoter, NOS terminator, 5-enolpyruvyl-shikimate-3-phosphate synthase gene(cp4-EPSPS) and soybean lectin gene were assayed by qualitative PCR, and the CaMV35S promoter countersigned with soybean lectin by aninternal reaction control was detedcted. Comparing the polyacrylamide gel electrophoresis with agarose gel electrophoresis in the PCR products analysis. The polyacrylamide gel electrophoresis was found to help improve PCR screen accuracy.

Key words: transgenic, GMO, PCR detection, electrophoresis