Abstract: Experiments on the beer yeast liquid sterilized by high-pressure carbon dioxide were carried out in this paper. Tests showed that, an obvious sterilizating effect on beer yeast was resulted. The sterilizating effects were strongly affected by the parameters, such as the pressure of the carbon dioxide, sterilizing time, the concentration of the beer yeast solution, and the diameter of the micro-pore from which the high pressure CO2 was spouted into the solution. The highest sterilizing efficiency was reached at 37.2% under the test conditions of 1.0MPa CO2 and 4 min sterilizing time.

Key words: sterilization, high-pressure, carbon dioxide, beer yeast