High Yield of Inulinase from Kluyveromyces S120 by Solid-state Fermentation

Abstract

Abstract: This paper firstly studied the high levels of inulinase by Kluyveromyces S120 under solid-state fermentation (SSF). The results showed that wheat bran was the best solid substrate, 10% inulin and 0.5% (NH4)2SO4 could enhance the yield of inulinase. The optimized process parameters were 20.0g medium in 300ml flask, initial moisture content of solid substrate 66%, initial pH of the medium 5.7, incubation temperature 34℃, inoculum size 3.9% and fermentation time 72h. The average yield in triplicate under optimal conditions was 118.28U/g(Dry Weight). Inulinase was produced in partial parallel with the strain growth, which made Kluyveromyces S120 promising for a potential application in industrial scale.

Key words: Kluyveromyces, solid-state fermentation, inulinase

CHEN Xiong, WANG Jin-Hua, LI Shi-Jie. High Yield of Inulinase from Kluyveromyces S120 by Solid-state Fermentation[J]. FOOD SCIENCE, 2005, 26(10): 54-57.

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High Yield of Inulinase from Kluyveromyces S120 by Solid-state Fermentation

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