He-Ne Laser Treatment of the Protoplast from Monascus ruber M9x

FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (11): 75-78.

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He-Ne Laser Treatment of the Protoplast from Monascus ruber M9x

DAI De-Hui, GUO Ai-Lian, JIANG Jia-Xin

1.School of Biological and Chemical Engineering, Zhejiang University of Science and Technology;2.Life School of Nowthwest University

Online:2005-11-15 Published:2011-10-01

Abstract

Abstract: A mutant strain called Monascus ruber M9y was obtained by using irradiating protoplasts with He-Ne laser (632. 8nm). Its yield of Monacolin K increased as much as 4.1 times than that of the original strain, to reach 103.73μg/ml. The esterase (EST) isoenzyme electropheresis bands of the mutant strain Monascus ruber M9y compared with that of the original strain changed dramatically. The stability test of Monascus ruber M9y from generation to generation was studied. Results showed that the yield of monacolin K was stable, whose mutation was not restored apparently after eight generations.

Key words: Monascus ruber, He-Ne laser, Monacolin K, isoenzymes

DAI De-Hui, GUO Ai-Lian, JIANG Jia-Xin. He-Ne Laser Treatment of the Protoplast from Monascus ruber M9x [J]. FOOD SCIENCE, 2005, 26(11): 75-78.

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He-Ne Laser Treatment of the Protoplast from Monascus ruber M9x

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