The Breeding of High-yield Pectinase Producing Baterial Using He-Ne Laser and Studies on Its Optimal Fermentation Conditions

Abstract

Abstract: A mutant strain ZH-2 with high yield of pectinase was selected by He-Ne laser mutagenesis on protoplasts of the original one. The optimal fermentation conditions were investigated: initial pH7.0, temperature 33℃, with 2% citrus peel powder and 1% lactose as carbon source, 1% (NH4)2SO4 and 0.3% yeast extract paste as nitrogen source, after fermenting for 32h on a rotary shaker. By preliminary analysis of the pectinase produced by ZH-2, optimum conditions for enzyme catalysis were pH6.5 at 50℃. It was stable and even retained 90% activity after incubation at 50℃ for 60min.

Key words: pectinase, He-Ne laser, mutagenesis, fermentation conditions, protoplasts

ZHU Hong-Li, SONG Ji-Rong, ZHANG Jia, HUANG Jie, XU Kang-Zhen. The Breeding of High-yield Pectinase Producing Baterial Using He-Ne Laser and Studies on Its Optimal Fermentation Conditions[J]. FOOD SCIENCE, 2005, 26(12): 160-164.

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The Breeding of High-yield Pectinase Producing Baterial Using He-Ne Laser and Studies on Its Optimal Fermentation Conditions

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