FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (12): 90-98.

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Separation, Purification and Structural Analysis of β-glucan from Oat

 WANG  Hai-Bo, XIE  Bi-Jun, LIU  Da-Chuan, WANG  Hai-Ying   

  1. 1.Food Science Department, Huazhong Agricultural University; 2.Basic Department, College of Tongji Medicine, Huazhong Science University
  • Online:2005-12-15 Published:2011-10-01

Abstract: Two kinds ofβ-glucan ,Oglu-1 and Oglu-2, were extract and purified from Oat. Oglu-1 was shown to be a single homogeneous component by GPC and Sephacryl-400SF column chromatography. It was found that Oglu-1 was composed of glucose units only and joined by β-D-(1→4) and β-D-(1→3) linkage with the linkage number ratio 2.1:1 by using the methods of paper chromatography, UV, IR, NMR and HLPC. The molecular average-weight of Oglu-1 is 4.49×105 was determined by CPC-LLS.

Key words: Oat, β-glucan, structural analysis